Journal: International journal of environmental research and public health

Article Title: The EDN1 Missense Variant rs5370 G > T Regulates Adaptation and Maladaptation under Hypobaric Hypoxia.

doi: 10.3390/ijerph191811174

Figure Lengend Snippet: Figure 2. EDN1 gene expression and ET1 plasma levels in the three study groups. (A) The relative expression of EDN1 was evaluated by RT PCR. The EDN1 expression was upregulated by 4.7-fold and 1.8-fold in the HAPE-p (n = 40) and HLs (n = 40) compared to HAPE-f (n = 40) (p < 0.001 and p = 0.03, respectively). (B) Plasma level of ET1, pg/ml was evaluated by immunoassay. ET-1 level was significantly higher in HAPE-p (n = 60) compared to HAPE-f (n = 60) and HLs (n = 60) (p = 0.03 and <0.001, respectively); (C) the risk allele T associated with the higher concentration of ET-1 in the three study groups compared to protective G allele (p < 0.05). (D,E) ROC curve exhibiting the significant AUC of 0.67 (0.59–0.75) and 0.93 (0.89–0.97) demonstrates the effect of ET-1 levels in HAPE-p (p < 0.001). Data are presented as mean ± SE and are compared by a two-tailed t-test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 was considered statistically significant. ROC, receiver operating characteristic curve; AUC, area under the curve; SE, standard error.

Article Snippet: Briefly, the wild-type (WT) EDN1 Lys198 plasmid (NM_001955, Origene Technology Inc., Rockville, MD, USA) was amplified using a pair of synthetic oligonucleotide primers containing the desired variant.

Techniques: Gene Expression, Clinical Proteomics, Expressing, Reverse Transcription Polymerase Chain Reaction, Concentration Assay, Two Tailed Test

Journal: International journal of environmental research and public health

Article Title: The EDN1 Missense Variant rs5370 G > T Regulates Adaptation and Maladaptation under Hypobaric Hypoxia.

doi: 10.3390/ijerph191811174

Figure Lengend Snippet: Figure 3. Both hypoxia and EDN1 rs5370T/Asn198 variant enhanced cellular ET1 levels. HEK293 cells were either transfected with one µg of pcDNA3.1 empty vector, control, or one µg of WT EDN1 G allele expressing plasmid or with one µg of Var EDN1 T allele expressing plasmid. Each of the transfected cells was either kept under (A) normoxic conditions for 30 h or (B) normoxia for 6 h, followed by hypoxic conditions (100 Mm CoCl2) for 24 h. Secreted ET-1 levels were highest in the presence of the risk T allele under normoxia and hypoxia. Data are presented as mean ± SE and are compared by a two-tailed t-test. **** p < 0.0001. WT, wild-type allele; Var, variant-type allele; SE, standard error.

Article Snippet: Briefly, the wild-type (WT) EDN1 Lys198 plasmid (NM_001955, Origene Technology Inc., Rockville, MD, USA) was amplified using a pair of synthetic oligonucleotide primers containing the desired variant.

Techniques: Variant Assay, Transfection, Plasmid Preparation, Control, Expressing, Two Tailed Test

Journal: The American Journal of Pathology

Article Title: Vascular Dysfunction in Pneumocystis -Associated Pulmonary Hypertension Is Related to Endothelin Response and Adrenomedullin Concentration

doi: 10.1016/j.ajpath.2015.10.008

Figure Lengend Snippet: ET-1 concentrations are not significantly different in BALB-STD or IFN-γ-STD mice compared with controls. Samples are peptide extracts from total lung homogenates. Data are expressed as means ± SEM. n = 5 mice in two independent experiments. CON, control; ET-1, endothelin-1; IFN, interferon; STD, short-term depletion.

Article Snippet: Polyclonal anti-endothelin receptor A (ET A R) and the control peptide to verify specificity were from Alomone Labs (no. AER-001; Jerusalem, Israel).

Techniques:

Journal: The American Journal of Pathology

Article Title: Vascular Dysfunction in Pneumocystis -Associated Pulmonary Hypertension Is Related to Endothelin Response and Adrenomedullin Concentration

doi: 10.1016/j.ajpath.2015.10.008

Figure Lengend Snippet: Small pulmonary artery profiles show increasing vasoconstriction when treated with a cumulative dose response of ET-1. Shown is a representative profile from a BALB-STD mouse precision cut lung slices sample. ET-1, endothelin-1; STD, short-term depletion.

Article Snippet: Polyclonal anti-endothelin receptor A (ET A R) and the control peptide to verify specificity were from Alomone Labs (no. AER-001; Jerusalem, Israel).

Techniques:

Journal: The American Journal of Pathology

Article Title: Vascular Dysfunction in Pneumocystis -Associated Pulmonary Hypertension Is Related to Endothelin Response and Adrenomedullin Concentration

doi: 10.1016/j.ajpath.2015.10.008

Figure Lengend Snippet: The response to ET-1 is enhanced in BALB-STD mice. A: Vasoconstriction in small pulmonary artery profiles in BALB-STD mice is significantly greater over the range of 10−10 to 10−9 ET-1. B: This coincides with a significant increase in the ETAR in BALB-STD mice total lung tissue as indicated by quantification of Western blot analysis. Data are expressed as means ± SEM. n = 4 to 5 (B) or n = 16 to 35 profiles per group across 4 to 5 mice (A) in two independent experiments. ∗P ≤ 0.05 versus IFN-γ-STD and controls. CON, control; ETAR, endothelin receptor A; ET-1, endothelin-1; IFN, interferon; STD, short-term depletion.

Article Snippet: Polyclonal anti-endothelin receptor A (ET A R) and the control peptide to verify specificity were from Alomone Labs (no. AER-001; Jerusalem, Israel).

Techniques: Western Blot